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Oleg G. Kisselev, Ph.D.
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Oleg G. Kisselev, Ph.D., Associate Professor Department of Ophthalmology Department of Biochemistry and Molecular Biology Saint Louis University School of Medicine 1755 S. Grand Blvd., Saint Louis, MO 63104 Phone: (314) 256-3253, Fax: (314) 771-2317 E-mail: kisselev@slu.edu EDUCATION: B.A. (Biochemistry, 1986), Ph.D (Biochemistry, 1990): Lomonosov Moscow State University Postdoctoral training: Washington University in St. Louis HONORS: 2002, William and Mary Greve Scholar Award from the Foundation for Research to Prevent Blindness. 1998, The International Society For Eye Research Fellow. 1994, American Heart Association Fellow. |
RESEARCH INTERESTS:
We are interested in the universal mechanism of transmembrane signal transduction mediated by heterotrimeric G-proteins, which are responsible for the transmission of information about extracellular messengers such as hormones, neurotransmitters and sensory stimuli, including visual signals.
Our laboratory is working on questions of the molecular organization of the receptor-G-protein complex, the high-resolution picture of the receptor-G-protein interface, the mechanism of signal transfer from the receptor, and the roles of individual G-protein subunits, especially the G-protein beta-gamma subunit complex, in this dynamic process.
The phototransduction cascade in highly specialized sensory neurons, retinal photoreceptor cells, is the prototypical G-protein-mediated system. As alterations of function of this system due to mutations, age-related processes or drug interactions may lead to severe visual disorders, one of our goals is the dissection of the phototransduction mechanism for clinical and therapeutic applications.
PUBLICATIONS:
Anderson M.A., Ogbay B., Kisselev O.G., Cistola D.P., Marshall G.R., Alternative Binding Mode of C-terminal Phenethylamine Analogs of Gta(340-350) to Photoactivated Rhodopsin, Chemical Biology and Drug Design, 2006, 68: 295-307.
Downs M.A., Arimoto, R., Marshall, G.R., Kisselev, O.G., G-protein alpha and beta-gamma subunits interact with conformationally distinct signaling states of rhodopsin. Vision Res., 2006, Dec: 46(27): 4442-8.
Kisselev O.G. and Downs M.A, Rhodopsin-interacting surface of transducin gamma subunit Biochemistry, 2006 Aug 8;45(31):9386-92.
Anderson M.A., Ogbay B., Arimoto R., Sha W., Kisselev O.G., Cistola D.P., and Marshall G.R., Relative strength of cation-pi vs. salt-bridge interactions: the Gta(340-350) peptide/rhodopsin system, J. Am. Chem. Soc., 2006, 128(23), 2006, pp. 7531-7541.
Kisselev O.G., Focus on Molecules: Rhodopsin. Exp. Eye Res., 2005 Oct;81(4):366-7.
Kisselev O.G., Downs M.A., McDowell J.H., Hargrave P.A., Conformational changes in the phosphorylated C-terminal domain of rhodopsin during rhodopsin arrestin interactions. J Biol Chem. 2004 Dec 3;279(49):51203-51207
Kisselev O.G., McDowell J.H., Hargrave P.A. The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin. FEBS Lett. 2004 Apr 30;564(3):307-11.
Kisselev O.G. and Downs M.A, Rhodopsin controls a conformational switch on transducin gamma-subunit. Structure, Cell press, 2003, 11, 367-373.
Arimoto, R., Kisselev, O.G., Makara, G.M., and Marshall, G.R., The rhodopsin-transducin interface: Studies with conformationally constrained peptides, 2001 Biophys J., 81(6):3285-93.
G. R. Marshall, R. Rango, G.M. Makara, R. Arimoto, and Kisselev, O.G., Bound conformations for ligands for G-protein coupled receptors; 1999, Lett. Pept. Science, 6:283-288.
Kisselev, O.G., Meyer, C.K., Heck, M., Ernst, O.P., Hofmann, K.P. Signal transfer from rhodopsin to the G-protein: Evidence for a two-site sequential fit mechanism. 1999; Proc. Natl. Acad. Sci. USA, 96, 4898-4903.
Kisselev, O.G., Kao, J., Ponder, J., Fann, Y., Gautam, N., and Marshall, G.R. Light activated rhodopsin induces structural binding motif in G-protein alpha subunit. 1998; Proc. Natl. Acad. Sci. USA, 95, 4270-4275.
Kisselev, O.G., Pronin, A.P., Gautam, N., Transient Expression of the beta-gamma-complex in fibroblasts and reconstitution assays with a receptor, rhodopsin. 1998, in G-proteins: Techniques of Analysis, Ed. David R. Manning, CRC Press, 85-98
Gautam, N., Yang, K., Downs, G., and Kisselev, O.G., G-protein beta-gamma-complex., 1998, Cell. Signal. 10(7):447-55
Kisselev, O.G., Ermolaeva, M.V., Gautam, N. Efficient interaction with a receptor requires a specific type of prenyl group on the G protein gamma subunit. J. Biol. Chem., 1995; 270 (43):25356-25358
Kisselev, O.G., Pronin, A.M., Ermolaeva, M.V., Gautam, N. Receptor-G protein coupling is established by a potential conformational switch in the beta-gamma complex. Proc. Natl. Acad. Sci. USA, 1995; 92:9102-9106
Kisselev, O.G., Ermolaeva, M.V., Gautam, N. A farnesylated domain in the G protein gamma subunit is a specific determinant of receptor coupling. J. Biol. Chem., 1994;269 (34):21399-21402
Kisselev, O.G., Gautam, N. Specific interaction with rhodopsin is dependent on the gamma subunit type in a G protein. J. Biol. Chem., 1993;268:24519-24522
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Oleg G. Kisselev, Ph.D.,
Associate Professor |
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Kisselev
kisselev@slu.edu EDUCATION: B.A. (Biochemistry, 1986), Ph.D (Biochemistry, 1990): Lomonosov Moscow State University Postdoctoral training: Washington University in St. Louis HONORS: 2002, William and Mary Greve Scholar Award from the Foundation for Research to Prevent Blindness. 1998, The International Society For Eye Research Fellow. 1994, American Heart Association Fellow. |
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